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加拿大萨尼亚论文代写:DNA提取实验

许多特定的技术被用于分离DNA样品。一些额外的染色体DNA更容易分离。这些是通过细胞裂解过程完成的,并且通过DNA的沉淀而被粘附。这些用于该过程以捕获染色体DNA。下一阶段是检测DNA。使用二苯胺(DPA)来确认DNA的存在。这些涉及化学水解的过程。 DNA浓度通过测量溶液吸光度的强度来确定。这是通过使用分光光度计来完成的。然后将它们与DNA浓度的标准曲线进行比较(Taberlet,Waits和Luikart,1999)。这些是基于DNA溶液吸光度的强度来衡量的。在这些情况下,DNA吸收260-280nm之间的紫外光。芳香族蛋白质约为280nm。

加拿大萨尼亚论文代写:DNA提取实验

DNA样本的纯度比例为1.8。通过用限制酶切割DNA的过程对DNA进行定量。然后使它们在琼脂糖凝胶下运行。它们用溴化乙锭或不同的污渍染色。这些与DNA的强度相比较。 DNA标记是已知浓度的标记。然后使用Southern印迹技术。这个过程是通过使用PCR或RFLP分析分离和检测的定量DNA完成的。随后开发这些程序以允许基因组中重复序列的区分。最终,用于DNA提取和分析过程的技术取决于实验目的和实验目的。

加拿大萨尼亚论文代写:DNA提取实验

Many specific techniques are used for the isolation of the DNA sample. Some of the extra chromosomal DNA is easier to isolate. These are done by the process of cell lysis and are adhered by the precipitation of the DNA. These are used for the process to trap chromosomal DNA. The next stage is the detection of the DNA. Use of diphenylamine (DPA) is used to confirm the presence of DNA. These involve the process of chemical hydrolysis. The DNA concentration is determined by the measure of the intensity of the absorbance of the solution. This is done by the use of the spectrophotometer. They are then compared to the standard curve of DNA concentrations (Taberlet, Waits and Luikart, 1999). These are measured based on the intensity of the absorbance of the DNA solutions. In these cases, the DNA absorbs the ultraviolet light between 260 to 280 nm. The aromatic proteins are about 280 nm.

加拿大萨尼亚论文代写:DNA提取实验

The purity of the sample of the DNA has the ratio of 1.8. The DNA is quantified by the process of cutting DNA with restriction enzymes. These are then made to run under the agarose gel. They are stained with ethidium bromide or different stain. These are compared with the intensity of DNA. The DNA markers are markers of the known concentration. Southern blot technique is then used. This process is done for the quantified DNA that is isolated and examined using PCR or RFLP analysis. These are then developed as procedures to allow the differentiation of repeated sequences in the genome. Ultimately, the techniques that are used for the process of DNA extraction and analysis depend on the objective of the experiment and the purpose of experimentation.